医药微生物技术

马莉

副教授
硕士生导师
个人简介 科研项目 学术论文 其他介绍

教育背景:

博士:2009.09-2013.01,毕业院校:中国科学院微生物研究所,专业:生物化学与分子生物学

硕士:2006.09-2009.06,毕业院校:中国海洋大学,专业:遗传学

学士:2002.09-2006.06,毕业院校:山西农业大学,专业:生物工程


工作经历:

2023.09至今,微生物技术国家重点实验室,山东大学,副教授

2019.03-2023.08,微生物技术国家重点实验室,山东大学,助理研究员

2013.07-2019.02,中国科学院青岛生物能源与过程研究所,助理研究员

2013.01-2013.06,国家海洋局第一海洋研究所,研究助理


研究方向:

1.酶工程,包括:工业酶资源的筛选、蛋白质进化改造、P450酶的结构功能研究

2.生物催化


主要项目:

1.国家自然科学基金面上项目,2021.01-2024.12,主持;

2.国家重点研发计划“变革性技术关键科学问题”重点专项子课题,2019.12-2024.11,主持;

3.山东大学基本科研业务费,2019.09-2021.12,主持;

4.青岛市应用基础研究计划项目,2017.05-2019.05,主持;

5.国家自然科学基金青年基金项目,2016.01-2019.12,主持;

6.山东省自然科学基金青年基金项目,2016.11-2019.06,主持。

代表性论文:

1.Qiu Z#, Liu X#, Yu J, Zhao Y, Zhao GR, Li S, Liu K*, Du L*,Ma L*. Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases. Synth Syst Biotechnol, 2024, 9(2):187-195.

2.Ma L, Sun T, Liu Y, Zhao Y, Liu X, Li Y, Chen X, Cao L, Kang Q, Guo J, Du L, Wang W, Li S*. Enzymatic synthesis of indigo derivatives by tuning P450 BM3 peroxygenases. Synth Syst Biotechnol, 2023, 8(3): 452-461.

3.Liu X#, Li F#, Sun T, Guo J, Zhang X, Zheng X, Du L, Zhang W,Ma L*, Li S. Three pairs of surrogate redox partners comparison for Class I cytochrome P450 enzyme activity reconstitution. Commun Biol, 2022, 5(1): 791.

4.Ma L, Li F, Zhang X, Chen H, Huang Q, Su J, Liu X, Sun T, Fang B, Liu K, Tang D, Wu D, Zhang W, Du L, Li S*. Development of MEMS directed evolution strategy for multiplied throughput and convergent evolution of cytochrome P450 enzymes. Sci China Life Sci, 2022, 65(3): 550-560.

5.Guo J, Li F, Cheng F,Ma L, Liu X, Durairaj P, Zhang G, Tang D, Long X, Zhang W, Du L, Zhang X*, Li S. Bacterial biosynthetic P450 enzyme PikCD50N: a potential biocatalyst for the preparation of human drug metabolites. J Org Chem, 2021, 86(21): 14563-14571.

6.You C, Li F, Zhang X,Ma L, Zhang YZ, Zhang W, Li S*. Structural basis for substrate specificity of the peroxisomal acyl-CoA hydrolase MpaH’involved in mycophenolic acid biosynthesis. FEBS J, 2021, 288, 5768-5780.

7.Li F,Ma L, Zhang X, Chen J, Qi F, Huang Y, Qu Z, Yao L, Zhang W, Kim ES, Li S.Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 fromSebekia benihana. Synth Syst Biotechnol, 2020, 5(3): 236-243.

8.Dong S, Chen J, Zhang X, Guo F,Ma L, You C, Wang X, Zhang W, Wan X, Liu SJ, Yao LS, Li S, Du L*, Feng Y*. Structural basis for selective oxidation of phosphorylated ethylphenolsby cytochrome P450 monooxygenase CreJ. Appl Environ Microbiol, 2021, 87: e00018-21.

9.Li Z, Jiang Y, Zhang X, Chang Y, Li S, Zhang X, Zheng S, Geng C, Meng P,Ma L, Yang Y, Gao Z, Tang YJ, Li S*. Fragrant venezuelaenes A and B with a 5−5−6−7 tetracyclic skeleton: discovery, biosynthesis, and mechanisms of central catalysts. ACS catalysis, 2020, 10, 5846-5851.

10.Li Z#, Jiang Y#, Guengerich FP,Ma L, Li S, Zhang W*. Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications. J Biol Chem. 2020, 295(3): 833-849.

11.Xu H, Liang W, Ning L, Jiang Y, Yang W, Wang C, Qi F,Ma L, Du L, Fourage L, Zhou Y J, Li S*. Directed evolution of P450 fatty acid decarboxylases via high-throughput screening towards improved catalytic activity. ChemCatChem, 2019, 11, 1-6.

12.Yao Q#,Ma L#, Liu L, Ikeda H, Fushinobu S, Li S, Xu LH*. Hydroxylation of Compactin (ML-236B) by CYP105D7 (SAV_7469) fromStreptomyces avermitilis. J Microbiol Biotechnol, 2017, 27(5): 956-964.

13.Sun Y#,Ma L#, Han D#, Du L, Qi F, Zhang W, Sun J, Huang S, Kim ES*, Li S*.In vitroreconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. J Ind Microbiol Biotechnol, 2017, 44(2): 161-166.

14.Du L,Ma L, Qi F, Zheng X, Jiang C, Li A, Wan X, Liu SJ*, Li S*. Characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation inCorynebacterium glutamicum. J Biol Chem, 2016, 291(12): 6583-6594.

15.Ma L, Du L, Chen H, Sun Y, Huang S, Zheng X, Kim ES, Li S*. Reconstitution of thein vitroactivity of the cyclosporine-specific P450 hydroxylase fromSebekia benihanaand development of a heterologous whole cell biotransformation system. Appl Environ Microbiol. 2015, 81(18), 6268-6275.

16.Li C#,Ma L#, Mou S, Wang Y, Zheng Z, Liu F, Qi X, An M, Chen H, Miao J*. Cyclobutane Pyrimidine Dimers Photolyase from Extremophilic Microalga: Remarkable UVB Resistance and Efficient DNA Damage Repair, Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2015, 773: 37-42.

17.Liu X#, Song F#,Ma L, Chen C, Xiao X, Ren B, Liu X, Dai H,Piggott AM, Av-Gay Y, Zhang L*, Capon RJ*. Sydowiols A–C:Mycobacterium tuberculosisprotein tyrosine phosphatase inhibitors from an East China Sea marine-derived fungus,Aspergillus sydowii. Tetrahedron Letters, 2013, 54(45):6081-6083.

18.Ma L, Liu X, Liang H, Che Y, Chen C, Dai H, Yu K, Liu M, Ma LY, Yang CH, Song F, Wang Y*, Zhang L*. Molecular targets of 14-alpha-lipoyl andrographolide on quorum sensing inPseudomonas aeruginosa. Antimicrob Agents Chemother, 2012, 56(12): 6088-6094.

19.Zeng X, Liu X, Bian J, Pei G, Dai H, Polyak SW, Song F,Ma L, Wang Y*, Zhang L*. Synergistic effect of AL-1 and various antibiotics on the formation of biofilms and production of exopolysaccharide and pyocyanin byPseudomonas aeruginosa.Antimicrobial Agents and Chemotherapy, 2011, 55(6): 3015-3017.

20.Ma L, Chen J*, Liu R, Zhang XH, Jiang YA. Mutation of rpoS gene decreased resistance to environmental stresses, synthesis of extracellular products and virulence ofVibrio anguillarum. FEMS Microbiol Ecol, 2009, 70(2): 130-136.


所获专利

1.李盛英,马莉,Eung-Soo Kim。一种制备毛发生长促进剂的生物转化方法(申请号:201510346350.8,申请公布号:CN106319007A,授权号:ZL201510346350.8)

2.缪锦来,许建方,马莉。一种南极冰藻CPD光修复酶、其编码基因和表达载体以及该酶的应用(申请号:201310121837.7,申请公布号:CN103160488A,授权号:ZL201310121837.7)