微生物学、生物化学与分子生物学

陈辉

教授
博士/硕士研究生导师
0532-58632496
个人简介 科研项目 学术论文 其他介绍

教育经历

博士起止时间:2010/09-2014/03 毕业院校:浙江大学专业:生物化工

硕士起止时间:2007/09-2010/03 毕业院校:浙江理工大学专业:应用化学

本科起止时间:2003/09-2007/06 毕业院校:鲁东大学专业:生物工程


工作经历

2022/09-至今山东大学教授

2021/07-2022/07 犹他大学化学系研究助理教授

2018/02-2021/06 犹他大学化学系博士后

2017/05-2018/02 明尼苏达大学双城分校博士后

2015/08-2017/05 弗吉尼亚理工大学博士后

2014/04-2015/06 中科院青岛生物能源与过程研究所助理研究员


研究方向

1.基于生物电催化的CO2固定,N2固定以及惰性化合物的深度转化

2.P450及其他具有工业应用价值酶的酶学与酶工程

3.电化学架构内合成生物学体系的构建与应用


研究方向

1.国家自然科学基金优秀青年基金(海外),2023-2025,主持

2.山东省自然科学基金优秀青年基金(海外),2023-2025,主持

3.山东省泰山学者(青年项目),2022-2026,主持

4.国家自然科学基金青年基金,2015-2017,主持

学术论文

1.N. Samali Weliwatte,#Hui Chen,#*Tianhua Tang, and Shelley D. Minteer* Three-Stage Conversion of Chemically Inert n‑Heptane to α‑Hydrazino Aldehyde Based on Bioelectrocatalytic C−H Bond Oxyfunctionalization.ACS Catalysis, 2022, DOI: 10.1021/acscatal.2c04003. (Accepted)

2.Burnett, J.W.#,Chen, H.#,Li, J., Li, Y., Huang, S., Shi, J., McCue, A.J., Howe, R.F., Minteer, S.D.* and Wang, X* Supported Pt Enabled Proton-Driven NAD(P)+Regeneration for Biocatalytic Oxidation.ACS Applied Materials & Interfaces, 2022, 14(18), 20943-20952.

3.Chen, H.,Tang, T., Malapit, C.A., Lee, Y.S., Prater, M.B., Weliwatte, N.S. and Minteer, S.D.* One-Pot Bioelectrocatalytic Conversion of Chemically Inert Hydrocarbons to Imines.Journal of American Chemical Society, 2022, 144 (9), 4047-4056. (封面论文)

4.Chen, H.#; Lin, Y.#; Long, Y. T.; Minteer, S.* Ying, Y. L.* Nanopore-based measurement of the interaction of P450cam monooxygenase and putidaredoxin at single-molecule level.Faraday Discussions,2022, 233, 295-302.

5.Dong, F.; Lee, Y. S.; Gaffney, E. M.; Grattieri, M.; Haddadin H.; Minteer, S. D.;Chen, H.*An engineered, non-diazotrophic cyanobacterium and its application in bioelectrochemical nitrogen fixation.Cell Reports Physical Science, 2021, 100444.

6.Chen, H.#; Simoska, O.#; Lim, K.; Grattieri, M.; Yuan M.; Dong, F.; Lee, Y.; Beaver, K.; Weliwatte, S.; Gaffney, E.; Minteer, S. D.* Fundamentals, Applications, and Future Directions of Bioelectrocatalysis.Chemical Reviews, 2020, 120(23), 12903-12993.(ACS Editors’ choice论文)

7.Dong, F.;Chen, H.*; Malapit, C. A.; Prater, M. B.; Li, M.; Yuan, M.; Lim, K.; Minteer, S. D.* Biphasic bioelectrocatalytic synthesis of chiral β-hydroxy nitriles.Journal of American Chemical Society, 2020, 142 (18), 8374-8382.

8.Chen, H.;Prater, M. B.; Cai, R.; Dong, F.; Chen, H.; Minteer, S. D.* Bioelectrocatalytic Conversion from N2to Chiral Amino Acids in a H2/α-Keto Acid Enzymatic Fuel Cell.Journal of American Chemical Society,2020, 142 (8), 4028-4036.

9.Chen, H.;Dong, F.; Minteer, S. D.* The progress and outlook of bioelectrocatalysis for the production of chemicals, fuels and materials.Nature Catalysis,2020, 3, 225-244.

10.Chen, H., Cai, R., Patel, J., Dong, F.Y., Chen, H., Minteer, S.*Upgraded Bioelectrocatalytic N2Fixation: From N2to Chiral Amine Intermediates.Journal of American Chemical Society,2019, 141 (12), 4963-4971.

11.Chen, H.*, Kim, E.J., Wu, C.H., Sun, J.S., Adams, M. W., Zhang, Y. H. P.* Building a thermostable metabolon for highly efficient cofactor transportation andin vitrobiohydrogen production at elevated temperature.ChemSusChem,2018, 11 (18), 3120-3130. (Backcover论文)

12.Chen, H., Meng X., Li, S.Y.* The molecular basis of lipase stereoselectivity.Applied Microbiology and Biotechnology7, 2018, 102 (8), 3487-3495.

13.Chen, H., Huang, R., Zhang, Y. H. P.* (2017).Systematic comparison of co-expression of multiple recombinant thermophilic enzymes inEscherichia coliBL21 (DE3).Applied Microbiology and Biotechnology, 2017, 101(11), 4481-4493.

14.Chen, H.#, Zhu, Z.G.#, Huang, R., Zhang, Y. H. P.* (2016).Coenzyme engineering of a hyperthermophilic 6-phosphogluconate dehydrogenase from NADP+to NAD+with its application to biobatteries.Scientific Reports, 2016, 6, 36311.

15.Chen, H.; Wu, J. P.*; Yang, L. R.*, Xu G. ImprovingPseudomonas alcaligeneslipase’s diastereopreference in hydrolysis of diastereomeric mixture of menthyl propionate by site-directed mutagenesis.Biotechnology and Bioprocess Engineering, 2014, 19(4), 592-604.

16.Chen, H., Wu, J. P.*, Yang, L. R.*, Xu G. Characterization and structure basis ofPseudomonas alcaligeneslipase’s enantiopreference towardsd,l-menthyl propionate.Journal of Molecular Catalysis B-Enzymatic, 2014, 102, 81-87.

17.Chen, H., Wu, J. P.*, Yang, L. R.*, Xu G. (2013). A combination of sit-directed mutagenesis and site-specific modification to improve diastereopreference ofPseudomonas alcaligeneslipase. Biochimica et Biophysica Acta, 2013, 1834, 2494-2501.

18.Chen H.; Yan M.; Zhu J.; Xu X.* Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures ofInonotus obliquusby lignocellulose decomposition.Journal of Industrial Microbiology & Biotechnology. 2011, 38(2), 291-298.

19.Chen H.; Xu X.*; Zhu Y. Optimization of hydroxyl radical scavenging activity of exopolysaccharides fromInonotus obliquusin submerged fermentation using response surface methodology.Journal of microbiology and biotechnology. 2010, 20(4), 835-43.

20.Lim K,; Macazo FC,; Scholes C,;Chen H.; Sumampong K,; Minteer SD.* Elucidating the mechanism behind the bionanomanufacturing of gold nanoparticles using Bacillus subtilis.ACS Applied Bio Materials, 2020, 3(6), 3859-3867.

21.Yuan M,; Abdellaoui S,;Chen H,; Kummer MJ,; Malapit CA,; You C,; Minteer SD.* Selective electroenzymatic oxyfunctionalization by alkane monooxygenase in a biofuel cell.Angewandte Chemie International Edition, 2020, 59, 8969-8973.

22.Patel J.; Cai R.; Milton R.;Chen H.; Minteer SD.* Pyrene‐Based Noncovalent Immobilization of Nitrogenase on Carbon Surfaces.ChemBioChem, 2020, 21, 1729-1732.

23.Grattieri M.;Chen H.; Minteer SD.* Chloroplast biosolar cell and self-powered herbicide monitoring.Chemical Communications. 2020, 56(86), 13161-13164.

24.Huang, R.*;Chen, H.; Upp, D. M.; Lewis, J. C.; Zhang, Y.-H. P. J.* A High-Throughput Method for Directed Evolution of NAD(P)+-Dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues.ACS Catalysis, 2019, 9 (12), 11709-11719.

25.Huang R.;Chen H.; Zhou W.; Ma C.; Zhang Y.H.* Engineering a thermostable highly active glucose 6-phosphate dehydrogenase and its application to hydrogen productionin vitro.Applied Microbiology and Biotechnology.2018, 102(7), 3203-3215.

26.Kim J.E.; Kim E.J.;Chen H.; Wu C.H.; Adams M.W.; Zhang Y.H*. Advanced water splitting for green hydrogen gas production through complete oxidation of starch by in vitro metabolic engineering.Metabolic Engineering, 2017, 44, 246-52.

27.Fang B.; Xu H.; Liu Y.; Qi F.; Zhang W.;Chen H.; Wang C.; Wang Y.; Yang W.; Li S.* Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT JE.Scientific Reports, 2017, 7(1), 1-7.

28.Huang, R.,Chen, H., Zhong, C., Kim, J. E., Zhang, Y. H. P. (2016). High-throughput screening of coenzyme preference change of thermophilic 6-phosphogluconate dehydrogenase from NADP+to NAD+.Scientific Reports, 2016, 32644.

29.Kim J.E.; Huang R.;Chen H.; You C.; Zhang Y.H.* Facile construction of random gene mutagenesis library for directed evolution without the use of restriction enzyme inEscherichia coli.Biotechnology Journal. 2016, 11(9), 1142-50.

30.Zheng X.; Fang B.; Han D.; Yang W.; Qi F.;Chen H.; Li S.* Improving the Secretory Expression of an α-Galactosidase fromAspergillus nigerinPichia pastoris.PloS one. 2016, 22, 11(8), e0161529.

31.Ma, L., Du, L.,Chen, H., Sun, Y., Huang, S. H., Zheng, X. L., Li, S.Y.(2015). Reconstitution of the in vitro activity of the cyclosporine-specific P450 hydroxylase fromSebekia benihanaand development of a heterologous whole-cell biotrasformation system.Applied and Environmental Microbiology, 2015, 81(18), 6268-6275.

32.Xu X.*, Wu Y,Chen H.Comparative antioxidative characteristics of polysaccharide-enriched extracts from natural sclerotia and cultured mycelia in submerged fermentation ofInonotus obliquus.Food Chemistry. 2011,127(1), 74-79.

人才称号

1.国家海外高层次青年人才

2.山东省海外高层次青年人才

3.山东省泰山学者青年专家

4.山东大学杰出中青年学者(第一层次)