教育背景

博士 起止时间：2013/09–2018/07 毕业院校：山东大学生命科学学院 专业：微生物学 硕博连读

学士 起止时间：2009/09–2013/07 毕业院校：青岛农业大学生命科学学院 专业：生物技术

工作经历

起止时间 单位名称 专业技术职务

2022/02–至今 山东大学微生物技术研究院 助理研究员

2018/06–2022/02山东大学微生物技术研究院 博士后

研究方向

1.复杂生物质多糖的降解机制解析

2.高性能酶的创制与协同优化

3.工业酶制剂活性和稳定性的精准设计

主要纵向项目：

2027.01-2030.12，嗜热几丁质酶在高结晶异质底物界面动态工作机制解析与智能设计，国家自然科学基金面上项目，32670033，主持

2025.01-2027.12，山东大学青年学者未来计划主持

2025.01-2027.12，基于结构组学的几丁质酶热适应催化机制解析与功能设计，山东省自然科学基金青年项目，ZR2024QC184，主持

2022.01-2024.12，嗜热几丁质酶SsChi18A持续性催化的分子机制研究，国家自然科学基金青年项目，32100022，主持

2020.10-2022.10，啤酒麦糟结构分析及木聚糖酶解转化工艺，啤酒生物发酵工程国家重点实验室开放课题，K202005，主持

2021.01-2022.12，高温碱性木聚糖酶的理性设计，绿色造纸全国重点实验室开放课题，GZKF202020，主持

主要横向项目：

1.益生微生物组学研究

2.合成生物学与微生物智造平台的搭建与应用

3.菌种鉴定与快速高通量分析技术研发

1. Sha Zhao, Feiyue Wen, Xiaomeng Sun, Yining Dong, Sheng Xing, Lushan Wang, Bo Ding* andXiuyun Wu*. Reconstruction of a cross-family chitinase cocktail for efficient chitobiose production guided by the secretome ofStreptomycessp. F-3.Food Chem.2026, 527: 150920.

2. Zhaoran Li, Zhixin Dou, Sha Zhao, Mengyu Liu,Xiuyun Wu*and Lushan Wang. Dual engineering of the hydrophobic core and functional loop reshapes the conformational energy landscape for significantly enhanced xylanase activity and thermostability.J. Agric. Food Chem.2026, 74(32): 25476–25489.

3. Zhaoran Li, Muyang Chen, Wenya Tian, Lushan Wang andXiuyun Wu*. Investigating the role of polar amino acids driven by evolution in the active site architecture of GH11 xylanase.Int. J. Biol. Macromol.2025, 315: 144464.

4. Sha Zhao, Zhaoran Li, Muyang Chen, Jingyi Zhu, Xukai Jiang,Xiuyun Wu*and Lushan Wang. Loop dynamics perturbation enhances the catalytic stability of GH18 family chitinases.J. Agric. Food Chem.2025, 73(32): 20450–20461.

5. Sha Zhao, Mengyu Liu, Xiaomeng Sun, Xukai Jiang, Yingjie Li,Xiuyun Wu*and Lushan Wang. Engineering the relatively conserved residues in active site architecture of thermophilic chitinaseSsChi18A enhanced catalytic activity.Biomacromolecules2024, 25(1): 238–247.

6.Xiuyun Wu, Sha Zhao, Zhennan Tian, Chao Han, Xukai Jiang and Lushan Wang*. Dynamics of loops surrounding the active site architecture in GH5_2 subfamily TfCel5A for cellulose degradation.Biotechnol. Biofuels Bioprod.2023, 16: 154.

7. Mengyu Liu, Shuxia Huang, Peng Yan, Hua Yin, Junhong Yu*,Xiuyun Wu*and Lushan Wang. Effective Degradation of Brewer Spent Grains by a Novel Thermostable GH10 Xylanase.Appl. Biochem. Biotechnol.2024, 196(8): 4837–4848.

8.Xiuyun Wu,Zelu Shi, Wenya Tian, Mengyu Liu, Shuxia Huang, Xinli Liu, Hua Yin* andLushan Wang*, A thermostable and CBM2-linked GH10 xylanase from

9.Xiuyun Wu, Shu Zhang, Sha Zhao, Lin Dai, Shuxia Huang, Xinli Liu, Junhong Yu,* and Lushan Wang*.Functional specificity of three αarabinofuranosidases from different glycoside hydrolase families inAspergillus nigerAn76.J. Agric. Food Chem.2022, 70:5039−5048.

10.Shu Zhang, Sha Zhao, Shang Weihao, Zijuan Yan,Xiuyun Wu*, Yingjie Li, Guanjun Chen, Xinli Liu, Lushan Wang. Synergistic mechanism of GH11 xylanases with different action modes fromAspergillus nigerAn76.Biotechnol Biofuels, 2021, 14(1): 118.

11.Xiuyun Wu, Shu Zhang, Qun Zhang, Yue Zhao, Guanjun Chen, Weihua Guo and Lushan Wang*.The contribution of specific subsites to catalytic activities in active site architecture of GH11 xylanase.Appl Microbiol Biotechnol. 2020,104: 8735–8745.

12.Xiuyun Wu, Qun Zhang, Lanzeng Zhang, Shijia Liu, Guanjun Chen, Huaiqiang Zhang and Lushan Wang*. Insights into the role of exposed surface charged residues in the alkali-tolerance of GH11 xylanase.Front Microbiol,2020,11: 872.

13.Xiuyun Wu, Zhennan Tian, Xukai Jiang, Qun Zhang, Lushan Wang*. Enhancement in catalytic activity ofAspergillus nigerXynB by selective site-directed mutagenesis of active site amino acids.Appl Microbiol Biotechnol, 2018, 102:249–260.

14.赵沙，颜子娟，张舒，余俊红，吴秀芸*，王禄山等。细菌几丁质酶结构、功能及分子设计的研究进展，生物化学与生物物理进展，2022，49(07):1179-1191.

15.张舒，赵越，陈冠军，余俊红，吴秀芸*，王禄山．生物质多糖的高效降解与降解酶（系）的精确定制，生物化学与生物物理进展2021，48(3):296-308.

16.吴秀芸，刘淑萍，赵越，陈冠军，王禄山*。快速展示糖苷水解酶活性架构单点突变导致结合力变化的电泳技术，生物化学与生物物理进展, 2018，45(5): 560~566.

17.吴秀芸，戴琳，张舒，尚伟昊，余俊红，黄小平，王禄山*。高效木聚糖降解酶系定制的新进展与挑战，微生物学通报, 2020, 47(7): 2278−2289.

1.王禄山，吴秀芸，刘诗佳，田震楠，张群，张怀强。一种木聚糖酶AnXynB的突变基因AnXynB_1及其应用，2019.0712，中国，ZL 201610498183.3。

2.王禄山，刘诗佳，吴秀芸，张怀强。一种木聚糖酶TlXynA的突变基因TlXynA_3及其应用，2019.0712，中国，ZL 201610497299.5。

3.王禄山，吴秀芸，戴琳，陈冠军，高培基。一种降解小麦阿拉伯木聚糖的复合酶系及其应用，2020.1030，中国，ZL201910589068.0。

4.王禄山，孙晓萌，赵越，张怀强，吴秀芸。一种突变的几丁质酶及其应用，2021.03.05，中国，ZL201911050815.X

5.王禄山，来玉红，吴秀芸。一株高效降解角蛋白的菌株及其应用，2021.09.22，中国，ZL 2021 1 1108104.0

6.王禄山，杨昊琳，吴秀芸，孙彩云。一株长枝木霉及其应用，2026.04.14，中国，ZL 2025 1 0100112.2